About the Facility
Accessing the Facility
Equipment and Services
Request Services
If you have projects that require glycomics assistance, but the techniques are not listed on this page, please fill out the request form.
Service | Technique/Equipment/Libraries | Description |
---|---|---|
Glycan Screening |
15 FT-ICR-MS |
|
Analytical R&D |
Nano-ES MALDI TOF/TOF 15 FT-ICR-MS |
|
Carbohydrate Synthesis |
Automated glycan synthesis (Glyconeer 2.1) Semipreparative chromatography |
|
Glycan-ligand Discovery |
Liquid Glycan Array (LiGA) Lectin microarray with dual and single-color analysis Catch-release MS assay Liposomal Nanoparticles assay (ZetaSizer) |
|
Glycomics Drug Discovery & High-Throughput Screening |
Fully automated and enclosed platform for cell-based and target-based screening High-content quantitative cell analysis (Cellomics VTi, Molecular Devices ImageXpress Micro Systems) Quantitative optical screening for cell-based kinetic assays (FLIPR) |
|
Biophysical & Structural Biology Analysis |
Microscale thermophoresis (Nanotempter Monolith) Nano ITC (TA instruments) Circular Dichroism (Jasco J-815 Spectropolarimeter) Protein crystallization with liquid handling robot (Mosquito Crystal Gryphon LCP) Mass spectrometry (Orbitrap Fusion Lumos Tribid Mass spectrometer, UPLC-SYNAPT G2 System) High-field NMR (Bruker 600 MHz equipped with helium cryoprobe) |
|
Molecular Glycobiology |
Streptomyces strains Plasmid constructs Molecular archives (cDNA, shRNA) Carbohydrate-active enzyme repository |
|
Tissue Culture & Cell Imaging |
Cell sorting (CyFlowTM Cube 6 V2m, BioSorter) Collection of cell lines (human, mammalian, transgenic mouse) Biosafety Level 2 Cell Culture Cabinets High-content microscope (Molecular Devices ImageXpress Micro Confocal Imaging System) |
|
Microbial Glycome Analysis |
GC-MS (Agilent Technology Ensemble 5977B) MALDI-TOF MS (Bruker UltrafleXtreme) NMR (Bruker Ascend TM600 Hyphenated) |
|
Glycoengineering |
Specialized E. coli strain with a dual plasmid system - optimization of glycoprotein expression In vitro enzymatic remodelling of protein glycans |
|
Canadian Glycomics Network
E5-33 Gunning/Lemieux Chemistry Centre
University of Alberta, T6G 2G2
Tel: 780-492-6204
Fax: 780-492-4147
© CANADIAN GLYCOMICS NETWORK (GLYCONET). ALL RIGHTS RESERVED.