Core Services
GlycoNet has centralized research facilities to provide rapid and economical access to pure research compounds for researchers’ needs. Our Synthetic Core Services have an extensive collection of pure, characterized carbohydrates and we provide expedient synthesis and analytical services with discounts to Network Investigators.
We furnish building blocks, glycans and fragments, specialized linkers, tailor-made molecules, oligosaccharide-protein conjugates, and much more. We accept projects from academic and industrial clients.
Collection of Carbohydrates and Enzymes
We have over 900 compounds in our collection, all of which are carefully synthesized and fully analyzed by NMR and mass spectrometry to assure purity and quality. Free compounds suitable for screening experiments are offered on an as-is basis, and we can provide further analysis, functionalization, and assembly into larger structures. Below are some examples of compounds in our collection:
Last updated: Feb 20, 2021
In addition to compounds, we have an inventory of carbohydrate-active enzyme plasmids, including glycosidases, transferases, kinases, mutases, synthases, and fluorescent proteins. Currently, over 75 constructs are in stock and are free for GlycoNet members.
Custom Synthesis
We supply our clients with custom-made molecules for their product development and project needs using our top-flight instruments and methods:- High field NMR spectrometers equipped with cryo-probes
- FIA-ESI, LC-ESI-MS, and MALCI mass spectrometers
- Normal phase and reverse phase preparative scale HLPC systems
- Large-scale rotary evaporation
- Automated peptide synthesis
- Enzyme expression and purification
Automated Glycan Assembly
Using Glyconeer 2.1, we offer our clients unparalleled access to a variety of glycan structures on a photo-cleavable solid support. Stepwise regiospecific chain elongation is achieved with standard glycosyl donor types and protecting groups. Building blocks (thioglycoside, imidate, phosphates) are sourced in-house or commercially on a cost-recovery basis. Excess building blocks and reagents may be recovered and recycled.
Photo-cleavage with 305 nm UV light in a flow reactor affords fully protected oligomers suitable for normal-phase high performance liquid chromatography. We use broad gradient elution conditions to isolate protected compounds from side products, ensuring subsequent global deprotection and functionalization start with pure materials.
Analytical Services
We provide services in extraction, purification, structure characterization, and binding interactions of all carbohydrates including polysaccharides, oligosaccharides, and glycoconjugates. We address any specific question by developing custom approaches with the following analytical technologies:- Routine characterization of oligosaccharide samples for QC and analysis
- Complex structure determination, including high-resolution conformational analysis and protein-carbohydrate complexes
- 1D/2D NMR (300, 400, 500, 600, 700 MHz), STD-NMR
- Compositional analysis (stoichiometry) of polysaccharides
- Oligosaccharide/Glycan sequencing
- Determination of unusual or modified glycan structures
- Nano-ES, MALDI TOF/TOF, FT-ICR
- Surface Plasma Resonance (BIAcore)
- Microcalorimetry
Molecular Modeling
Our team is specialized in molecular dynamics of glycoproteins and protein-carbohydrate complexes using various software and tools including GLYCAM and Gaussian. Contact us for any specific topic. We can study any question on the base of a non-disclosure agreement.
Ligand Discovery
48Hour Discovery Inc. is a spin-off company in February 2017 with the support of GlycoNet. Using a proprietary platform technology, 48Hour Discovery rapidly sorts through libraries containing billions of genetically-encoded peptides to find the best candidates, data, and information for targeted drug development. The company provides open data accessibility for researchers in academia and industry that other platform competitors do not provide.
For more information or to request a quote, please contact the Core Services at coreservices@glyconet.ca. Please mention whether you are a GlycoNet member to receive special rates for our services.
Canadian Glycomics Network
E5-33 Gunning/Lemieux Chemistry Centre
University of Alberta, T6G 2G2
Tel: 78-492-6204
Fax: 780-492-4147
© CANADIAN GLYCOMICS NETWORK (GLYCONET). ALL RIGHTS RESERVED.
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